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This project has received funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement no 613678

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Strategies to develop effective, innovative and practical approaches to protect major european fruit crops from pests and pathogens

Trunk injection - Novel delivery system of antimicrobial peptides

Picture: (Production of) Lactobacillus plantarum PM411

The synthetic linear undecapeptide BP100 (KKLFKKILKYL-NH2) has potent bactericidal activity against bacterial plant pathogens Xanthomonas arboricola pv. pruni (bacterial spot of stonefruit), Pseudomonas syringae pv. actinidiae (bacterial canker of kiwifruit) and Xanthomonas fragariae (angular leaf spot of strawberry). The peptide has been synthetized in prototype formulation and was effective in the control of the bacterial diseases in greenhouse and semi-field assays. The efficacy was comparable to antibiotics.

Semi-industrial scale production of a strain of Lactobacillus plantarum PM411 and Bacillus amyloliquefaciens EPS2017 was succesful, and a prototype formulation showed significant efficacy in the control of the three bacterial diseases in greenhouse and semi-field testing.


Endotherapy

Endotherapy

Picture: Trunk injection

A novel system for delivery of antimicrobial peptides, by trunk injection has been validated and was effective in controlling X. arboricola pv. pruni on peach and P. syringae pv. actinidiae on kiwifruit potted plants in the greenhouse. Experiments are being done to validate the technology in the field.

Author

Author

Emilio Montesinos
University of Girona