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This project has received funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement no 613678

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Strategies to develop effective, innovative and practical approaches to protect major european fruit crops from pests and pathogens

Antimicrobial compounds against bacterial diseases


























Production of Lactobacillus plantarum TC92, an efficient antagonist of plant pathogenic bacteria


Biodegradable small synthetic antimicrobial peptides have proven to be effective against the three bacterial pathogens: Pseudomonas syringae pv. actinidiae (Psa); Xanthomonas fragariae (Xf), and X. arboricola pv. pruni (Xap). A number of selected strains of lactic acid bacteria isolated from fruit trees were effective in preventing infections by the bacterial pathogens. On the bases of preliminary toxicity tests these products are considered as safe.

Antimicrobials

Antimicrobials

In this project the first prototype formulations of two lead products; i.e. the antimicrobial peptide BP100 and the strains of Lactobacillus plantarum PM411 and TC92 have been prepared.










Production of the first batch of a formulated prototype antimicrobial peptides BP100 and BP143 from the CECMEL11 linear undecapeptide library for plant tests of bacterial disease control (University of Girona)

Control of bacterial pathogens

Control of bacterial pathogens

These products were tested against of the bacterial pathogens Xap, Psa and Xf, on respectively peach, kiwifruit and strawberry plants, under controlled environment greenhouse conditions. The effect of these products was similar to conventional antibiotics.

The next stages will be to scale-up the production, and to be tested under field conditions in orchards.







Testing of prototype formulations of Lactobacillus plantarum PM411 and antimicrobial peptide BP100 on kiwifruit, peach and strawberry plants in the quarantine controlled environment greenhouse (University of Girona)

Author

Author

Emilio Montesinos
University of Girona